feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine

نویسندگان

fariba sadeghi fazel health management department, quality assurance deputy, razi vaccine & serum research institute, karaj, iran

mahnaz haddadi cancer models research center, cancer institute of iran, tehran university of medical sciences, tehran, iran

alireza khoshnevisan department of neurosurgery, shariati hospital, tehran university of medical sciences, tehran, iran

samad muhammadnejad research center for molecular and cellular imaging, tehran university of medical sciences, tehran, iran

چکیده

objective(s):p in vitro chemosensitivity and resistance assays (csras) are a promising tool for personalized treatment of glioblastoma multiform (gbm). these assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. we aimed to assess the feasibility and validity of utilizing stereotactic biopsies of gbm in csras. materials and methods: single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of gbm. also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[aga1] . results: there was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods. conclusion: this work supports the feasibility of using stereotactic biopsies of gbm in csras.

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Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine

Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. Materials and Methods: Single...

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Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine

OBJECTIVES P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. MATERIALS AND METHODS Single ...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۷، شماره ۱۱، صفحات ۹۲۲-۹۲۵

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